Mapping and analysis of Pho85-Pcl1 phosphorylation websites on Shs1. (A) The distribution of in vitro and in vivo phosphorylation websites on Shs1, and a summary of the phosphorylation sites mutated in shs1-ps mutants
Two unbiased digestions (trypsin and chymotrypsin) have been performed on Shs1 gel bands, which...