He CBP/p300 drug experiment and also the extract was administered as single dose andHe experiment

He CBP/p300 drug experiment and also the extract was administered as single dose and
He experiment as well as the extract was administered as single dose and observed for the mortality up to 48 h study period (short term toxicity). According to the short term toxicity profile, the next dose of the extract was determined as per OECD recommendations No.420. The maximum dose tested (2000 mg/kg) for LD50. From the LD50, doses like 1/20th, 1/10th and 1/5th have been chosen and regarded as as low, medium and high dose i.e., 100 mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats were divided into 5 groups of 6 rats in each and every. The group I serves as normal control received automobile (CMC two in typical saline ten ml/kg b.wt), the group II received Furosemide (ten mg/kg, p.o) in car; other groups III, IV, V have been treated with low, medium, and high doses of alcoholic extract of roots of Cissampelos pareira in automobile and instantly right after the extract treatment all the rats were hydrated with saline (15 ml/kg) and placed within the metabolic cages (2 per cage), specially made to separate urine and faeces andS. no. 1 2 three four five groups Handle (10 ml/Kg b. wt) Regular (Frusemide ten mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (one hundred mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira Higher (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals supply a natural safeguard against illnesses and are a substantial remedy for particular illnesses. Diuretics have proved to be extremely important within the therapy of mild to moderate hypertension and also in enhancing the effect of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These agents are beneficial in decreasing volume over load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They decrease plasma volume and subsequently venous return for the heart. This decreases the cardiac function load, oxygen demand and plasma volume and also decreases blood pressure. Thusna+ mmol/l 113.03 + 2.16 191.05+2.09 129.40+2.*** ***total urine Vol (ml/kg b.wt/5 h) 13.45.02 22.23.01 15.20.*** ***K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1.*** ***Cl- mmol/l 82.95 + 1.42 129.06+1.67*** 94.42 + 1.73*** 109.44+1.20*** 121.39+2.00***17.41.02*** 20.46.***164.99+2.00*** 184.53+2.***77.93+2.67*** 85.11+1.***[Table/Fig-1]: Impact of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as mean S.E.M.,n=6, Significance at p0.05*, p0.01**, p0.001***, Compared with handle group (1 Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Investigation. 2014 May well, Vol-8(five): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, Bradykinin B2 Receptor (B2R) Accession carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in distinct plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with the majority of these plant phytochemical substances pointed out above. Hence it may be reported that the observed diuretic activity is as a consequence of these above phytoconstituents.CONCLUSIONResults showed that single dos.